Application
Antibody used in WB on Transfected 293T at 0.2-1 ug/ml.| Product Name | RFX1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RFX1, EF-C, EFC, RFX |
| Product description | RFX1 is a member of the regulatory factor X protein family, which are transcription factors that contain a highly-conserved winged helix DNA binding domain. RFX1 is structurally related to regulatory factors X2, X3, X4, and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with RFX family members X2, X3, and X5, but not with X4. This protein binds to the X-boxes of MHC class II genes and is essential for their expression. Also, it can bind to an inverted repeat that is required for expression of hepatitis B virus genes. This gene is a member of the regulatory factor X gene family, which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by this gene is structurally related to regulatory factors X2, X3, X4, and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with RFX family members X2, X3, and X5, but not with X4. This protein binds to the X-boxes of MHC class II genes and is essential for their expression. Also, it can bind to an inverted repeat that is required for expression of hepatitis B virus genes. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.1) Wang, K.R., (2007) J. Biol. Chem. 282 (36), 26167-26177. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RFX1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| RFX1 antibody can be used for detection of RFX1 by ELISA at 1:62500. RFX1 antibody can be used for detection of RFX1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RFX1 |
|---|---|
| Alternative Names | RFX1 EF-C EFC RFX |
| Molecular Weight(MW) | 105 kDa |
Application
Antibody used in WB on Transfected 293T at 0.2-1 ug/ml.| Positive Control | 1) Tranfected 293T Cell Lysate |
|---|---|
| Application Notes | RFX1 antibody can be used for detection of RFX1 by ELISA at 1:62500. RFX1 antibody can be used for detection of RFX1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store RFX1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
