Application
Immunocytochemistry of RFX1 in HeLa cells with RFX1 at 5 μg/mL.Application
Western blot analysis of RFX1 in HeLa cell lysate with RFX1 antibody at (A) 1 and (B) 2 μg/ml.| Product Name | RFX1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RFX, MHC class II regulatory factor RFX, MHC class II regulatory factor RFX1, enhancer factor C, EFC, trans-acting regulatory factor 1, transcription factor RFX1 |
| Product description | RFX1 is a member of the regulatory factor X protein family, which are transcription factors that contain a highly-conserved winged helix DNA binding domain and the D region found in the C-terminal part of these proteins which facilitates dimerization (1,2). RFX1 is structurally related to regulatory factors X2, X3, X4, and X5 (3). RFX1 is a regulatory factor essential for MHC class II genes expression and can bind to an inverted repeat that is required for expression of hepatitis B virus genes (4). Recent study identifies RFX1 may play a tumor suppressor role in HCC as autophagy mediator (5).1) Reith W, Ucla C, Barras E, et al. RFX1, a transactivator of hepatitis B virus enhancer I, belongs to a novel family of homodimeric and heterodimeric DNA-binding proteins. Mol. Cell Biol. 1994; 14:1230-44. |
| Immunogen | RFX1 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human RFX1. The immunogen is located within the last 50 amino acids of RFX1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCWB |
| RFX1 antibody can be used for detection of RFX1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RFX1 |
|---|---|
| Alternative Names | RFX MHC class II regulatory factor RFX MHC class II regulatory factor RFX1 enhancer factor C EFC trans-acting regulatory factor 1 transcription factor RFX1 |
| Molecular Weight(MW) | Predicted: 108 kDa Observed: 125 kDa |
| Sequence Similarities | 2) Cat. No. 17-001 - HeLa Cell Slide |
| Tissue Specificity | RFX1 antibody is human specific. At least two isoforms of RFX1 are known to exist; this antibody will detect both isoforms. |
Application
Immunocytochemistry of RFX1 in HeLa cells with RFX1 at 5 μg/mL.
Application
Western blot analysis of RFX1 in HeLa cell lysate with RFX1 antibody at (A) 1 and (B) 2 μg/ml.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | RFX1 antibody can be used for detection of RFX1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | RFX1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | RFX1 antibody is supplied in PBS containing 0.02% sodium azide. |
