Application
Antibody used in WB on Human 721_B at 0.2-1 ug/ml.| Product Name | RFX2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RFX2, FLJ14226, |
| Product description | RFX2 is a member of transcription factors that contain a highly-conserved winged helix DNA binding domain. RFX2 is structurally related to regulatory factors X1, X3, X4, and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with other RFX family members. This protein can bind to cis elements in the promoter of the IL-5 receptor alpha gene.This gene is a member of the regulatory factor X gene family, which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by this gene is structurally related to regulatory factors X1, X3, X4, and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with other RFX family members. This protein can bind to cis elements in the promoter of the IL-5 receptor alpha gene. Two transcript variants encoding different isoforms have been described for this gene, and both variants utilize alternative polyadenylation sites.1) Horvath, G.C., (2004) Biol. Reprod. 71 (5), 1551-1559. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RFX2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| RFX2 antibody can be used for detection of RFX2 by ELISA at 1:1562500. RFX2 antibody can be used for detection of RFX2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RFX2 |
|---|---|
| Alternative Names | RFX2 FLJ14226 |
| Molecular Weight(MW) | 80 kDa |
Application
Antibody used in WB on Human 721_B at 0.2-1 ug/ml.| Positive Control | 1) 721_B Cell Lysate |
|---|---|
| Application Notes | RFX2 antibody can be used for detection of RFX2 by ELISA at 1:1562500. RFX2 antibody can be used for detection of RFX2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store RFX2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
