Application
Western blot analysis in 293 cell line lysates (35ug/lane).| Product Name | RFX5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DNA-binding protein RFX5, Regulatory factor X 5, RFX5 |
| Product description | A lack of MHC-II expression results in a severe immunodeficiency syndrome called MHC-II deficiency, or the bare lymphocyte syndrome (BLS; MIM 209920). At least 4 complementation groups have been identified in B-cell lines established from patients with BLS. The molecular defects in complementation groups B, C, and D all lead to a deficiency in RFX, a nuclear protein complex that binds to the X box of MHC-II promoters. The lack of RFX binding activity in complementation group C results from mutations in the RFX5 gene encoding the 75-kD subunit of RFX (Steimle et al., 1995). RFX5 is the fifth member of the growing family of DNA-binding proteins sharing a novel and highly characteristic DNA-binding domain called the RFX motif. Multiple alternatively spliced transcript variants have been found but the full-length natures of only two have been determined. [provided by RefSeq].1) Laird, K.M., et al. J. Mol. Biol. 403(1):40-51(2010) |
| Immunogen | This RFX5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 237-266 amino acids from the Central region of human RFX5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RFX5 |
|---|---|
| Alternative Names | DNA-binding protein RFX5 Regulatory factor X 5 RFX5 |
| Molecular Weight(MW) | 65 kDa |
Application
Western blot analysis in 293 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
