Application
Western blot analysis in NCI-H292 cell line lysates (35ug/lane).| Product Name | RGL4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Ral-GDS-related protein, hRGR, Ral guanine nucleotide dissociation stimulator-like 4, RalGDS-like 4, RGL4, RGR |
| Product description | Ral GDS (Ral guanine nucleotide dissociation stimulator) is a guanine nucleotide exchange factor (GEF) that activates Ral and is implicated in oncogenic Ras-induced cell transformation. RGL4 (ral guanine nucleotide dissociation stimulator-like 4), also known as Rgr, is a 473 amino acid protein that localizes to the cytoplasmic vesicle of cells. Belonging to the GEF family of proteins, RGL4 induces phosphorylation of ERKs, p38 and JNK kinases, and it increases the levels of GTP bound forms of Ral and Ras. Ras activation is crucial for the transforming activity of RGL4. Due to its similarity to Ral GDS, RGL4 may be implicated in carcinogenesis.1) Hernandez-Munoz, I., et al. Cancer Res. 63(14):4188-4195(2003) |
| Immunogen | This RGL4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 98-125 amino acids from the N-terminal region of human RGL4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RGL4 |
|---|---|
| Alternative Names | Ral-GDS-related protein hRGR Ral guanine nucleotide dissociation stimulator-like 4 RalGDS-like 4 RGL4 RGR |
| Molecular Weight(MW) | 52 kDa |
Application
Western blot analysis in NCI-H292 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
