The protein encoded by RGS13 is a member of the regulator of G protein signaling (RGS) family. RGS family members share similarity with S. cerevisiae SST2 and C. elegans egl-10 proteins, which contain a characteristic conserved RGS domain. RGS proteins accelerate GTPase activity of G protein alpha-subunits, thereby driving G protein into their inactive GDP-bound form, thus negatively regulating G protein signaling. RGS proteins have been implicated in the fine tuning of a variety of cellular events in response to G protein-coupled receptor activation. The biological function of this gene, however, is unknown.1) Islam, T.C., et al., (2003) Leukemia 17 (9), 1880-1890.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RGS13.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
RGS13 antibody can be used for detection of RGS13 by ELISA at 1:62500. RGS13 antibody can be used for detection of RGS13 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human Daudi at 0.2-1 ug/ml.
Positive Control
1) Cat. No. 1224 - Daudi Cell Lysate
Application Notes
RGS13 antibody can be used for detection of RGS13 by ELISA at 1:62500. RGS13 antibody can be used for detection of RGS13 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store RGS13 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.