Application
Western blot analysis in U937 cell line lysates (35ug/lane).| Product Name | RGS14 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Regulator of G-protein signaling 14, RGS14, RGS14 |
| Product description | This gene encodes a member of the regulator of G-protein signaling family. This protein contains one RGS domain, two Raf-like Ras-binding domains (RBDs), and one GoLoco domain. The protein attenuates the signaling activity of G-proteins by binding, through its GoLoco domain, to specific types of activated, GTP-bound G alpha subunits. Acting as a GTPase activating protein (GAP), the protein increases the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G-protein heterotrimers, thereby terminating the signal. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.1) Wang, J., et al. Carcinogenesis 31(10):1755-1761(2010) |
| Immunogen | This RGS14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 223-251 amino acids from the Central region of human RGS14. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RGS14 |
|---|---|
| Alternative Names | Regulator of G-protein signaling 14 RGS14 RGS14 |
| Molecular Weight(MW) | 61 kDa |
Application
Western blot analysis in U937 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
