Application
Immunocytochemistry of RGS21 in HepG2 cells with RGS21 antibody at 2.5 μg/mL.Application
Immunofluorescence of RGS21 in HepG2 cells with RGS21 antibody at 20 μg/mL.Application
Western blot analysis of RGS21 in HepG2 cell lysate with RGS21 antibody at 0.5 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | RGS21 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RGS21 Antibody: Regulator of G-protein signaling 21, RGS21 |
| Product description | RGS21 Antibody: Regulator of G-protein signaling (RGS) proteins contain an 120 amino acid conserved domain, termed the RGS domain, that acts as a GTPase-activating protein that acts to reduce the signal transmitted by the receptor-activated G-alpha subunit. RGS21 is a recently identified member of this family that has been reported to be selectively expressed in subpopulations of taste bud cells and co-expressed with bitter and sweet transduction components such as alpha-gusticin, phospholipase Cbeta2, T1R2/T1R3 sweet taste receptors and T2R bitter taste receptors. Other reports indicate that RGS21 is more widely expressed. Binding assays demonstrate that RGS21 binds alpha-gusticin in a conformation-dependent manner and may do the same with T1R receptors, suggesting that RGS21 may play a role in sweet and bitter taste transduction processes.1) De Vries L, Mousli M, Wurmser A, et al. GAIP, a protein that specifically interacts with the G protein G alpha i3, is a member of a protein family with a highly conserved core domain. Proc. Natl. Acad. Sci. USA1995; 92:11916-20. |
| Immunogen | RGS21 antibody was raised against a 16 amino acid synthetic peptide near the center of human RGS21. The immunogen is located within amino acids 40 - 90 of RGS21. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| RGS21 antibody can be used for detection of RGS21 by Western blot at 0.5 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RGS21 |
|---|---|
| Alternative Names | RGS21 Antibody: Regulator of G-protein signaling 21 RGS21 |
| Sequence Similarities | 2) Cat. No. 17-011 - Hep G2 Cell Slide |
| Entrez Gene | 431704 |
|---|---|
| Protein Accession | NP_001034241 |
Application
Immunocytochemistry of RGS21 in HepG2 cells with RGS21 antibody at 2.5 μg/mL.
Application
Immunofluorescence of RGS21 in HepG2 cells with RGS21 antibody at 20 μg/mL.
Application
Western blot analysis of RGS21 in HepG2 cell lysate with RGS21 antibody at 0.5 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | RGS21 antibody can be used for detection of RGS21 by Western blot at 0.5 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | RGS21 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | RGS21 Antibody is supplied in PBS containing 0.02% sodium azide. |
