Application
Western blot analysis of RGS22 in Jurkat cell lysate with RGS22 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of RGS22 in human testis tissue with RGS22 antibody at 20 μg/mL.| Product Name | RGS22 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | RGS22 Antibody: Regulator of G-protein signaling (RGS) proteins contain an 120 amino acid conserved domain, termed the RGS domain, that acts as a GTPase-activating protein that acts to reduce the signal transmitted by the receptor-activated G-alpha subunit. RGS22 is a recently identified member of this family that localizes to the testis and can interact with guanine nucleotide binding proteins alpha 11, 12, and 13 (GNA11, GNA12, and GNA13). While RGS22 has been postulated to play a role in spermiogenesis in the testis, it is also expressed in several cancer cell lines with an epithelial origin and associated with cancer metastasis. Its overexpression in a highly metastatic cancer causes a decrease in cell migration and a reduction of the invasive potential of the cells, suggesting that RGS22 may be a potential prognostic biomarker for metastasis.1) De Vries L, Mousli M, Wurmser A, et al. GAIP, a protein that specifically interacts with the G protein G alpha i3, is a member of a protein family with a highly conserved core domain. Proc. Natl. Acad. Sci. USA 1995; 92:11916-20. |
| Immunogen | RGS22 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human RGS22. The immunogen is located within amino acids 870 - 920 of RGS22. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISA,IF,WB |
| RGS22 antibody can be used for detection of RGS22 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | Human,Mouse,Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RGS22 |
|---|---|
| Alternative Names | RGS22 Antibody: CT145 PRTD-NY2 Regulator of G-protein signaling 22 RGS22 |
| Molecular Weight(MW) | 139 kDa |
| Sequence Similarities | 2) Cat. No. 11-701 - Human Testis Tissue Slide |
| Tissue Specificity | At least four isoforms of RGS22 are known to exist; this antibody will detect the three longest isoforms. RGS22 antibody is predicted to not cross-react with other RGS proteins |
Application
Western blot analysis of RGS22 in Jurkat cell lysate with RGS22 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of RGS22 in human testis tissue with RGS22 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | RGS22 antibody can be used for detection of RGS22 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | RGS22 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | RGS22 Antibody is supplied in PBS containing 0.02% sodium azide. |
