Rgs7 inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Activity on G (o)-alpha is specifically enhanced by the RGS6/GNG5 dimer. It may play an important role in the rapid regulation of neuronal excitability and the cellular responses to short-lived stimulations and in synaptic vesicle exocytosis1) Haraguchi, S., (2003) Mech. Dev. 120 (6), 721-731.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse Rgs7.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
Rgs7 antibody can be used for detection of Rgs7 by ELISA at 1:312500. Rgs7 antibody can be used for detection of Rgs7 by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Mouse NIH-3T3 at 5.0 ug/ml.
Positive Control
1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Application Notes
Rgs7 antibody can be used for detection of Rgs7 by ELISA at 1:312500. Rgs7 antibody can be used for detection of Rgs7 by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store Rgs7 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt