Application
Antibody used in WB on Human HepG2 at 1.25 ug/ml.| Product Name | RHAG Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RHAG, RH2, RH50A, Rh50, Rh50 GP, CD241, Rh50GP, SLC42A1 |
| Product description | The Rh blood group antigens are associated with human erythrocyte membrane proteins of approximately 30 kD, the so-called Rh30 polypeptides. Heterogeneously glycosylated membrane proteins of 50 and 45 kD, the Rh50 glycoproteins, are coprecipitated with the Rh30 polypeptides on immunoprecipitation with anti-Rh-specific mono- and polyclonal antibodies. The Rh antigens appear to exist as a multisubunit complex of CD47, LW, glycophorin B, and play a critical role in the Rh50 glycoprotein.The Rh blood group antigens (MIM 111700) are associated with human erythrocyte membrane proteins of approximately 30 kD, the so-called Rh30 polypeptides. Heterogeneously glycosylated membrane proteins of 50 and 45 kD, the Rh50 glycoproteins, are coprecipitated with the Rh30 polypeptides on immunoprecipitation with anti-Rh-specific mono- and polyclonal antibodies. The Rh antigens appear to exist as a multisubunit complex of CD47 (MIM 601028), LW (MIM 111250), glycophorin B (MIM 111740), and play a critical role in the Rh50 glycoprotein.[supplied by OMIM].1) Norberg, A., (2006) Neurosci. Lett. 396 (2), 137-142. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RHAG. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| RHAG antibody can be used for detection of RHAG by ELISA at 1:312500. RHAG antibody can be used for detection of RHAG by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | RHAG |
|---|---|
| Alternative Names | RHAG RH2 RH50A Rh50 Rh50 GP CD241 Rh50GP SLC42A1 |
| Molecular Weight(MW) | 45 kDa |
Application
Antibody used in WB on Human HepG2 at 1.25 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | RHAG antibody can be used for detection of RHAG by ELISA at 1:312500. RHAG antibody can be used for detection of RHAG by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store RHAG antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
