Application
Western blot analysis of RHBDD1 in K562 cell lysate with RHBDD1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | RHBDD1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RHBDD1 Antibody: RRP4, RHBDL4, HSD-50, HSD50, Rhomboid-related protein 4, Rhomboid domain-containing protein 1, RRP4 |
| Product description | RHBDD1 Antibody: The Rhomboid family of proteins is made up of several widely conserved polytopic membrane serine proteases that play roles in growth and development. RHBDD1 is highly expressed in the testis and is involved in the cleavage of BIK, a proapoptotic member of the Bcl-2 family. Overexpression or suppression by RNAi of RHBDD1 in 293 cells will reduce or enhance BIK-mediated apoptosis, respectively, demonstrating that RHBDD1 modulates BIK-mediated apoptotic activity. In GC-1 cells, a spermatogonia cell line that can differentiate into spermatids within the seminiferous tubules, suppression of RHBDD1 expression by RNAi caused the cells to lose the ability to survive and differentiate in mouse seminiferous tubules, suggesting that RHBDD1 may be associated with mammalian spermatogenesis.1) Koonin EV, Makarova KS, Rogozin IB, et al. The rhomboids: a nearly ubiquitous family of intramembrane serine proteases that probably evolved by multiple horizontal gene transfers. Genome Biol.2003; 4:R19. |
| Immunogen | RHBDD1 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human RHBDD1. The immunogen is located within the last 50 amino acids of RHBDD1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| RHBDD1 antibody can be used for detection of RHBDD1 by Western blot at 1 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RHBDD1 |
|---|---|
| Alternative Names | RHBDD1 Antibody: RRP4 RHBDL4 HSD-50 HSD50 Rhomboid-related protein 4 Rhomboid domain-containing protein 1 RRP4 |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat: (86%) |
Application
Western blot analysis of RHBDD1 in K562 cell lysate with RHBDD1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1204 - K562 Cell Lysate |
|---|---|
| Application Notes | RHBDD1 antibody can be used for detection of RHBDD1 by Western blot at 1 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | RHBDD1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | RHBDD1 Antibody is supplied in PBS containing 0.02% sodium azide. |
