Application
Western blot analysis of RHBDD3 in rat lung tissue lysate with RHBDD3 antibody at 1 μg/mL.Application
Immunofluorescence of RHBDD32 in rat lung tissue with RHBDD3 antibody at 20 μg/mL.| Product Name | RHBDD3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RHBDD3 Antibody: PTAG, C22orf3, HS984G1A, Rhomboid domain-containing protein 3 |
| Product description | RHBDD3 Antibody: The Rhomboid family of proteins is made up of several widely conserved polytopic membrane serine proteases that play roles in growth and development. RHBDD3 was initially identified as a novel pituitary tumor apoptosis gene (PTAG) whose expression was reduced in certain pituitary adenomas. Overexpression of RHBDD3 in AtT20 cells showed an increase in apoptotic activity and caspase activation in response to bromocriptine, and apoptosis-inducing dopamine D2 analog, suggesting that reactivation of RHBDD3 in tumors may be a therapeutically useful tool. Later experiments also showed that RHBDD3 expression is also reduced in several primary colorectal tumors, indicating that loss of RHBDD3 contributes to a blunted apoptotic response and probably predisposes cells towards malignant transformation.1) Koonin EV, Makarova KS, Rogozin IB, et al. The rhomboids: a nearly ubiquitous family of intramembrane serine proteases that probably evolved by multiple horizontal gene transfers. Genome Biol.2003; 4:R19. |
| Immunogen | RHBDD3 antibody was raised against a 13 amino acid synthetic peptide from near the center of human RHBDD3. The immunogen is located within amino acids 170 - 220 of RHBDD3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| RHBDD3 antibody can be used for detection of RHBDD3 by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RHBDD3 |
|---|---|
| Alternative Names | RHBDD3 Antibody: PTAG C22orf3 HS984G1A Rhomboid domain-containing protein 3 |
Application
Western blot analysis of RHBDD3 in rat lung tissue lysate with RHBDD3 antibody at 1 μg/mL.
Application
Immunofluorescence of RHBDD32 in rat lung tissue with RHBDD3 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1462 - Rat Lung Tissue Lysate |
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| Application Notes | RHBDD3 antibody can be used for detection of RHBDD3 by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | RHBDD3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | RHBDD3 Antibody is supplied in PBS containing 0.02% sodium azide. |
