RHBDF1 is a seven-transmembrane protein with a long N-terminal cytoplasmic extension that comprises half of the polypeptide sequence, and is found in the endoplasmic reticulum and Golgi, but not on the cell surface. RHBDF1 has a pivotal role in sustaining growth signals in epithelial cancer cells and thus may serve as a therapeutic target for treating epithelial cancers.1) Oh, J.H., (2005) Mamm. Genome 16 (12), 942-954.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RHBDF1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
RHBDF1 antibody can be used for detection of RHBDF1 by ELISA at 1:62500. RHBDF1 antibody can be used for detection of RHBDF1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human 721_B at 0.2-1 ug/ml.
Positive Control
1) 721_B Cell Lysate
Application Notes
RHBDF1 antibody can be used for detection of RHBDF1 by ELISA at 1:62500. RHBDF1 antibody can be used for detection of RHBDF1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store RHBDF1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.