RHBDL2 is a member of the rhomboid family of integral membrane proteins. This family contains proteins that are related to Drosophila rhomboid protein. Members of this family are found in both prokaryotes and eukaryotes and are thought to function as intramembrane serine proteases. RHBDL2 is thought to release soluble growth factors by proteolytic cleavage of certain membrane-bound substrates, including ephrin B2 and ephrin B3.The protein encoded by this gene is a member of the rhomboid family of integral membrane proteins. This family contains proteins that are related to Drosophila rhomboid protein. Members of this family are found in both prokaryotes and eukaryotes and are thought to function as intramembrane serine proteases. The encoded protein is thought to release soluble growth factors by proteolytic cleavage of certain membrane-bound substrates, including ephrin B2 and ephrin B3.1) Gregory, S.G., (2006) Nature 441 (7091), 315-321.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RHBDL2.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
WB
RHBDL2 antibody can be used for detection of RHBDL2 by ELISA at 1:312500. RHBDL2 antibody can be used for detection of RHBDL2 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human PANC1 at 0.2-1 ug/ml.
Positive Control
1) PANC1 Cell Lysate
Application Notes
RHBDL2 antibody can be used for detection of RHBDL2 by ELISA at 1:312500. RHBDL2 antibody can be used for detection of RHBDL2 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store RHBDL2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.