Application
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
RHBG Antibody IHC analysis in formalin fixed and paraffin embedded mouse kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis of RHBG Antibody in K562 cell line lysates (35ug/lane)| Product Name | RHBG Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Ammonium transporter Rh type B, Rhesus blood group family type B glycoprotein, Rh family type B glycoprotein, Rh type B glycoprotein, RHBG |
| Product description | RHBG encodes one of two non-erythroid members of the Rhesus (Rh) protein family. Non-erythroid Rh protein family members are mainly expressed in the kidney and belong to the methylammonium-ammonium permease/ammonia transporters superfamily. All Rh family proteins are predicted to be transmembrane proteins with 12 membrane spanning domains and intracytoplasmic N- and C-termini.1) Han, K.H., et al. Am. J. Physiol. Lung Cell Mol. Physiol. 297 (1), L153-L163 (2009) |
| Immunogen | This RHBG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 423-450 amino acids from the C-terminal region of human RHBG. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RHBG |
|---|---|
| Alternative Names | Ammonium transporter Rh type B Rhesus blood group family type B glycoprotein Rh family type B glycoprotein Rh type B glycoprotein RHBG |
| Molecular Weight(MW) | 47 kDa |
Application
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
RHBG Antibody IHC analysis in formalin fixed and paraffin embedded mouse kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis of RHBG Antibody in K562 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
