Application
Antibody used in WB on Human MCF-7 at 0.2-1 ug/ml.| Product Name | RHCE Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RHCE, CD240CE, MGC103977, RH, RH30A, RHC, RHE, RHIXB, RHPI, Rh4, RhIVb(J), RhVI, RhVIII |
| Product description | The Rh blood group system is the second most clinically significant of the blood groups, second only to ABO. It is also the most polymorphic of the blood groups, with variations due to deletions, gene conversions, and missense mutations. The Rh blood group includes this gene which encodes both the RhC and RhE antigens on a single polypeptide and a second gene which encodes the RhD protein. The classification of Rh-positive and Rh-negative individuals is determined by the presence or absence of the highly immunogenic RhD protein on the surface of erythrocytes. A mutation in this gene results in amorph-type Rh-null disease.The Rh blood group system is the second most clinically significant of the blood groups, second only to ABO. It is also the most polymorphic of the blood groups, with variations due to deletions, gene conversions, and missense mutations. The Rh blood group includes this gene which encodes both the RhC and RhE antigens on a single polypeptide and a second gene which encodes the RhD protein. The classification of Rh-positive and Rh-negative individuals is determined by the presence or absence of the highly immunogenic RhD protein on the surface of erythrocytes. A mutation in this gene results in amorph-type Rh-null disease. Alternative splicing of this gene results in four transcript variants encoding four different isoforms.1) Cotorruelo, C.M., (er) BMC Med. Genet. 9 (1), 40 (2008) In press. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RHCE. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| RHCE antibody can be used for detection of RHCE by ELISA at 1:62500. RHCE antibody can be used for detection of RHCE by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RHCE |
|---|---|
| Alternative Names | RHCE CD240CE MGC103977 RH RH30A RHC RHE RHIXB RHPI Rh4 RhIVb(J) RhVI RhVIII |
| Molecular Weight(MW) | 29 kDa |
Application
Antibody used in WB on Human MCF-7 at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1219 - MCF7 Cell Lysate |
|---|---|
| Application Notes | RHCE antibody can be used for detection of RHCE by ELISA at 1:62500. RHCE antibody can be used for detection of RHCE by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store RHCE antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
