ELISA
Coat the plate with immunizing antigen.Blue line: Immune serum; Orange line: Pre-immune serum.ICC/IF
Immunofluorescence analysis of U251 cells using RHCE antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.Cells are fixed in 4% paraformaldehyde at room temperature for 15 minutes. Then, they are permeabilized with a PBS (OM750003) solution containing 0.1% Triton X-100(OM750021) at room temperature for 15 minutes. Subsequently, the cells are blocked with 10% non - immune goat serum(OM760028) at room temperature for 1 hour.The cells are incubated overnight at 4°C with the primary antibody diluted 1:100 in PBS. The secondary antibody, Omnimabs® 488 Goat Ant-Rabbit IgG(H&L) (Green,OM643486), is diluted at a ratio of 1:400 and incubated with the cells for 1 hour.Nuclear DNA is labeled with DAPI (Blue,OM643160). F-actin is stained with Omnimabs® 594-Phalloidin (Red,OM750007) diluted 1:100 for 30 minutes.| Product Name | Anti-RHCE antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IF |
| ICC/IF:1:100-1:500 ELISA:1:1000-1:10000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | RHCE |
|---|---|
| Gene Synonyms | CD240CE RH RH30A Rh4 RHC RHCe(152N) RHE RhIVb(J) RHIXB RHNA RHPI RhVI RhVIII SLC42A4 |
| Gene Full Name | Rh blood group CcEe antigens |
| Gene Summary | The Rh blood group system is the second most clinically significant of the blood groups, second only to ABO. It is also the most polymorphic of the blood groups, with variations due to deletions, gene conversions, and missense mutations. The Rh blood group includes this gene which encodes both the RhC and RhE antigens on a single polypeptide and a second gene which encodes the RhD protein. The classification of Rh-positive and Rh-negative individuals is determined by the presence or absence of the highly immunogenic RhD protein on the surface of erythrocytes. A mutation in this gene results in amorph-type Rh-null disease. Alternative splicing of this gene results in multiple transcript variants encoding several different isoforms. [provided by RefSeq, Aug 2016] |
| Alternative Names | (C)ces type 1 Rhesus blood group D antigen blood group protein RHCE blood group Rh(CE) polypeptide 144G RHCE blood group Rh(CE) polypeptide 225A RHCE blood group Rh(CE) polypeptide 341A RHCE blood group Rh(CE) polypeptide 347G RHCE blood group Rh(CE) polypeptide 537G RHCE blood group Rh(CE) polypeptide 658C RHCE blood group Rh(CE) polypeptide 718G RHCE blood group Rh(CE) polypeptide 999A RHCE blood group RhCcEe antigen blood group RhCE polypeptide conventional Rhce blood group protein Rh blood group antigen Evans Rh blood group C antigen Rh blood group CcEe antigen Rh blood group CcEe antigen cE_340T Rh blood group CcEe antigens RHCE_916G Rh blood group CE antigen Rh blood group D antigen Rh blood group protein Rh polypeptide rh polypeptide 1 Rh polypeptide I RHCE RhCE blood group antigen RhCE blood group antigens RHCE blood group variant Crawford antigen Rh43 RhCE protein CE antigen RHCE*cE_340T RHCE*CeCw blood group antigen RhD antigen Rhesus blood group antigen CE rhesus blood group antigen RhC antigen Rhesus blood group CcEe antigen Rhesus blood group CE protein Rhesus blood group E antigen rhesus blood group little e antigen Rhesus blood group Rhce antigen rhesus C/E antigens Rhesus system C and E polypeptides silenced Rh blood group CcEe antigen |
| Molecular Weight(MW) | 45kDa |
| Source | Rabbit |
| Cellular Localization | Membrane |
| SwissProt ID | P18577 |
|---|
ELISA
Coat the plate with immunizing antigen.Blue line: Immune serum; Orange line: Pre-immune serum.
ICC/IF
Immunofluorescence analysis of U251 cells using RHCE antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.Cells are fixed in 4% paraformaldehyde at room temperature for 15 minutes. Then, they are permeabilized with a PBS (OM750003) solution containing 0.1% Triton X-100(OM750021) at room temperature for 15 minutes. Subsequently, the cells are blocked with 10% non - immune goat serum(OM760028) at room temperature for 1 hour.The cells are incubated overnight at 4°C with the primary antibody diluted 1:100 in PBS. The secondary antibody, Omnimabs® 488 Goat Ant-Rabbit IgG(H&L) (Green,OM643486), is diluted at a ratio of 1:400 and incubated with the cells for 1 hour.Nuclear DNA is labeled with DAPI (Blue,OM643160). F-actin is stained with Omnimabs® 594-Phalloidin (Red,OM750007) diluted 1:100 for 30 minutes.| Application Notes | ICC/IF:1:100-1:500 ELISA:1:1000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300,40% Glycerol. |
