Application
Immunohistochemistry of Rheb in mouse brain tissue with Rheb antibody at 2 μg/mL.Application
Western blot analysis of Rheb in mouse brain cell lysate with Rheb antibody at (A) 1, (B) 2, and (C) 4 μg/mL.Application
Immunofluorescence of Rheb in Mouse Brain cells with Rheb antibody at 20 μg/mL.| Product Name | Rheb Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Rheb Antibody: RHEB2, RHEB2, GTP-binding protein Rheb, Ras homolog enriched in brain |
| Product description | Rheb Antibody: Rheb (Ras homolog enriched in brain) is an evolutionarily conserved member of the Ras family of small GTP-binding proteins originally found to be rapidly induced by synaptic activity in the hippocampus following seizure. While it is expressed at relatively high levels in the brain, Rheb is widely expressed in other tissues and may be induced by growth factor stimulation. Similar to other family members, Rheb triggers activation of the Raf-MEK-MAPK pathway. Biochemical and genetic studies demonstrate that Rheb has an important role in regulating the insulin/Target of rapamycin (TOR) signaling pathway. TOR is a serine/threonine protein kinase that acts as a sensor for ATP and amino acids, balancing the availability of nutrients with protein translation and cell growth. A dimeric protein complex termed TSC1/TSC2 indirectly inhibits TOR activity by inhibiting Rheb via the GAP activity of TSC2.1) Yamagata K, Sanders LK, Kaufman WE, et al. rheb, a growth factor- and synaptic activity-regulated gene, encodes a novel Ras-related protein. J. Biol. Chem. 1994; 269:16333-9. |
| Immunogen | Rheb antibody was raised against a 15 amino acid synthetic peptide from the middle region of human Rheb. The immunogen is located within amino acids 40 - 90 of Rheb. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| Rheb antibody can be used for the detection of Rheb by Western blot at 1 to 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RHEB |
|---|---|
| Alternative Names | Rheb Antibody: RHEB2 RHEB2 GTP-binding protein Rheb Ras homolog enriched in brain |
| Molecular Weight(MW) | 19, 20 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (100%) |
Application
Immunohistochemistry of Rheb in mouse brain tissue with Rheb antibody at 2 μg/mL.
Application
Western blot analysis of Rheb in mouse brain cell lysate with Rheb antibody at (A) 1, (B) 2, and (C) 4 μg/mL.
Application
Immunofluorescence of Rheb in Mouse Brain cells with Rheb antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1403 - Mouse Brain Tissue Lysate |
|---|---|
| Application Notes | Rheb antibody can be used for the detection of Rheb by Western blot at 1 to 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Rheb antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Rheb Antibody is supplied in PBS containing 0.02% sodium azide. |
