Application
Antibody used in WB on Jurkat 4.0 ug/ml (Lane A: Primary Antibody and Lane B: Primary Antibody + Blocking Peptide ).Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.Application
Antibody used in IHC on Human Intestine at 4.0-8.0 ug/ml.| Product Name | RHOBTB1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RHOBTB1, KIAA0740, MGC33059, MGC33841 |
| Product description | RHOBTB1 belongs to the Rho family of the small GTPase superfamily. It contains a GTPase domain, a proline-rich region, a tandem of 2 BTB (broad complex, tramtrack, and bric-a-brac) domains, and a conserved C-terminal region. The protein plays a role in small GTPase-mediated signal transduction and the organization of the actin filament system.The protein encoded by this gene belongs to the Rho family of the small GTPase superfamily. It contains a GTPase domain, a proline-rich region, a tandem of 2 BTB (broad complex, tramtrack, and bric-a-brac) domains, and a conserved C-terminal region. The protein plays a role in small GTPase-mediated signal transduction and the organization of the actin filament system. Alternate transcriptional splice variants have been characterized.1) Beder, L.B., (2006) J. Cancer Res. Clin. Oncol. 132 (1), 19-27. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RHOBTB1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| RHOBTB1 antibody can be used for detection of RHOBTB1 by ELISA at 1:312500. RHOBTB1 antibody can be used for detection of RHOBTB1 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RHOBTB1 |
|---|---|
| Alternative Names | RHOBTB1 KIAA0740 MGC33059 MGC33841 |
| Molecular Weight(MW) | 77 kDa, 79 kDa, 79 kDa |
Application
Antibody used in WB on Jurkat 4.0 ug/ml (Lane A: Primary Antibody and Lane B: Primary Antibody + Blocking Peptide ).
Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.
Application
Antibody used in IHC on Human Intestine at 4.0-8.0 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | RHOBTB1 antibody can be used for detection of RHOBTB1 by ELISA at 1:312500. RHOBTB1 antibody can be used for detection of RHOBTB1 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store RHOBTB1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
