Application
Immunohistochemistry of RHOG in mouse kidney tissue with RHOG antibody at 5 μg/ml.Application
Western blot analysis of RHOG in human kidney tissue lysate with RHOG antibody at 1 μg/ml.WB
(D) KTN1, RAC1, RHOA, CDC42, RHOG, and β-actin expression was evaluated in RT4 and T24 cells transfected with sh-KTN1, KTN1-AS1, and KTN1-AS1+sh-KTN1 by Western blot.| Product Name | RHOG Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The ras homolog family member G (RHOG), also known as rho-related GTP-binding protein, is a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades (1). RHOG controls a pathway that requires the microtubule network and activates Rac1 and Cdc42Hs independently of their growth factor signaling pathway (2). RHOG is also involved in the transcriptional regulation of interferon-gamma and nuclear factor of activated T cells (NFAT) and the regulation of the actin skeleton in lymphocytes (3).1) Vincent S, Janteur P, and Fort P. Growth-regulated of rhoG, a new member of the ras homolog family. Mol. Cell Biol. 1992; 12:3138-48. |
| Immunogen | RHOG antibody was raised against a 15 amino acid peptide near the amino terminus of human RHOG. The immunogen is located within amino acids 40 - 90 of RHOG. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| RHOG antibody can be used for detection of RHOG by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RHOG |
|---|---|
| Alternative Names | Ras homolog family member G Rho-related GTP-binding protein ARHG |
| Molecular Weight(MW) | Predicted: 21 kDa Observed: 23 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (87%), Guinea pig: (87%), Chicken: (87%), Pig: (71%) |
| Tissue Specificity | RHOG antibody is human, mouse and rat reactive. |
Application
Immunohistochemistry of RHOG in mouse kidney tissue with RHOG antibody at 5 μg/ml.
Application
Western blot analysis of RHOG in human kidney tissue lysate with RHOG antibody at 1 μg/ml.
WB
(D) KTN1, RAC1, RHOA, CDC42, RHOG, and β-actin expression was evaluated in RT4 and T24 cells transfected with sh-KTN1, KTN1-AS1, and KTN1-AS1+sh-KTN1 by Western blot.| Positive Control | 1) Cat. No. 1305 - Human Kidney Tissue Lysate |
|---|---|
| Application Notes | RHOG antibody can be used for detection of RHOG by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | RHOG antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | RHOG antibody is supplied in PBS containing 0.02% sodium azide. |
