Homeobox proteins are transcription factors notable for their ability to regulate embryogenesis. The reproductive homeobox proteins (Rhox) are expressed in a cell type-specific manner; several are hormonally regulated, and their expression pattern during postnatal testis development corresponds to their chromosomal position. Most of the Rhox proteins are expressed in Sertoli cells, the nurse cells in direct contact with developing male germ cells, suggesting that they regulate the expression of somatic-cell gene products critical for germ cell development.1) Maclean, J.A. (2005) Cell 120 (3), 369-382.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse RHOX11.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
WB
RHOX11 antibody can be used for detection of RHOX11 by ELISA at 1:312500. RHOX11 antibody can be used for detection of RHOX11 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Mouse SP2/0 at 0.2-1 ug/ml.
Positive Control
1) SP2/0 Cell Lysate
Application Notes
RHOX11 antibody can be used for detection of RHOX11 by ELISA at 1:312500. RHOX11 antibody can be used for detection of RHOX11 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store RHOX11 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.