Application
Antibody is used in Western blot to detect RPN1 in HeLa cell lysate (Lane 1) and mouse liver tissue lysate (Lane 2).Application
Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | Ribophorin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit, Ribophorin I, RPN-I, Ribophorin-1, RPN1 |
| Product description | Ribophorins 1 and 2 are abundant and highly conserved glycoproteins residing in the endoplasic reticulum, that participate in ribosome binding. Mammalian oligosaccharyltransferase activity is associated with a protein complex composed of RPN1, RPN2, and an oligosaccharyltransferase protein. RPN1 is a component of the proteasome base. The ubiquitin-like (UBL) domain of recombinant Rad23 interacts with proteasomes through the leucine-rich repeat domain of RPN1. The RPN1 gene maps to chromosome 3 in somatic cell hybrids, and the RPN2 gene maps to chromosome 20 by in situ hybridization.1) Fu, J., et al., J. Biol. Chem. 275(6):3984-3990 (2000). Pekarsky, Y., et al., Cancer Res. 57(18):3914-3919 (1997). Crimaudo, C., et al., EMBO J. 6(1):75-82 (1987). |
| Immunogen | This Ribophorin (RPN1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 531-561 amino acids from the C-terminal region of human Ribophorin (RPN1). |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Symbol | RPN1 |
|---|---|
| Alternative Names | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit Ribophorin I RPN-I Ribophorin-1 RPN1 |
| Molecular Weight(MW) | 69 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Monkey |
Application
Antibody is used in Western blot to detect RPN1 in HeLa cell lysate (Lane 1) and mouse liver tissue lysate (Lane 2).
Application
Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
