Application
RICH2 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis in MCF-7 cell line lysates (35ug/lane).| Product Name | RICH2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Rho GTPase-activating protein 44, NPC-A-10, Rho-type GTPase-activating protein RICH2, RhoGAP interacting with CIP4 homologs protein 2, RICH-2, ARHGAP44, KIAA0672, RICH2 |
| Product description | GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. Acts as a GTPase activitor in vitro for CDC42 and RAC1.1) Rollason, R., et al. J. Cell Biol. 184(5):721-736(2009) |
| Immunogen | This RICH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 784-811 amino acids from the C-terminal region of human RICH2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ARHGAP44 |
|---|---|
| Alternative Names | Rho GTPase-activating protein 44 NPC-A-10 Rho-type GTPase-activating protein RICH2 RhoGAP interacting with CIP4 homologs protein 2 RICH-2 ARHGAP44 KIAA0672 RICH2 |
| Molecular Weight(MW) | 89 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat |
Application
RICH2 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis in MCF-7 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
