Application
Immunocytochemistry of RILP in HeLa cells with RILP antibody at 2.5 μg/mL.Application
Immunofluorescence of RILP in HeLa cells with RILP antibody at 20 μg/mL.Application
Western blot analysis of RILP in A20 cell lysate with RILP antibody at 1 μg/mL.| Product Name | RILP Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RILP Antibody: PP10141, Rab-interacting lysosomal protein |
| Product description | RILP Antibody: The Rab interacting lysosomal protein (RILP) is lysosomal protein that interacts with RAB7, a small GTPase that controls transport to endocytic degradative compartments. It is thought that RILP is a downstream effector of RAB7 and both proteins act together in the regulation of late endocytic traffic. Overexpression of RILP caused enlarged lysosomes that are more centrally located in the cell suggesting that RILP may also be involved in the regulation of lysosomal morphology. Other evidence suggests that RILP may play a role in the biogenesis of multivesicular bodies.1) Cantalupo G, Alifano P, Roberti V, et al. Rab-interacting lysosomal protein (RILP): the Rab7 effector required for transport to lysosomes. EMBO J. 2001; 20:683-93. |
| Immunogen | RILP antibody was raised against an 18 amino acid synthetic peptide near the center of human RILP. The immunogen is located within amino acids 110 - 160 of RILP. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| RILP antibody can be used for detection of RILP by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RILP |
|---|---|
| Alternative Names | RILP Antibody: PP10141 Rab-interacting lysosomal protein |
| Sequence Similarities | 2) Cat. No. 17-001 - HeLa Cell Slide |
Application
Immunocytochemistry of RILP in HeLa cells with RILP antibody at 2.5 μg/mL.
Application
Immunofluorescence of RILP in HeLa cells with RILP antibody at 20 μg/mL.
Application
Western blot analysis of RILP in A20 cell lysate with RILP antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1288 - A20 Cell Lysate |
|---|---|
| Application Notes | RILP antibody can be used for detection of RILP by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | RILP antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | RILP Antibody is supplied in PBS containing 0.02% sodium azide. |
