Application
Immunofluorescence of RILPL1 in mouse brain cells with RILPL1 antibody at 20 μg/mL.Application
Western blot analysis of RILPL1 in rat cerebellum tissue lysate with RILPL1 antibody at (A) 0.5 and (B) 1 μg/mL.| Product Name | RILPL1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | RILPL1 Antibody: While the function of the Rab interacting lysosomal protein-like 1 (RILPL1) remains unknown, the homologous protein RILP is a small GTPase that controls transport to endocytic compartments, suggesting RILPL1 may have similar functions. However, overexpression of RILP caused enlarged lysosomes that are more centrally located in the cell, while overexpression of RILPL1 had no visible effect on the location or morphology of lysosomes.1) Cantalupo G, Alifano P, Roberti V, et al. Rab-interacting lysosomal protein (RILP): the Rab7 effector required for transport to lysosomes. EMBO J. 2001; 20:683-93 |
| Immunogen | RILPL1 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human RILPL1. The immunogen is located within the last 50 amino acids of RILPL1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISA,IF,WB |
| RILPL1 antibody can be used for detection of RILPL1 by Western blot at 0.5 and 1 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | Human,Mouse,Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RILPL1 |
|---|---|
| Alternative Names | RILPL1 Antibody: RLP1 GOSPEL RLP1 RILP-like protein 1 Rab-interacting lysosomal-like protein 1 |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (94%) |
| Tissue Specificity | At least three isoforms of RILPL1 are known to exist; this antibody will detect the two shorter isoforms. RILPL1 antibody is predicted to not cross-react with RILP. |
Application
Immunofluorescence of RILPL1 in mouse brain cells with RILPL1 antibody at 20 μg/mL.
Application
Western blot analysis of RILPL1 in rat cerebellum tissue lysate with RILPL1 antibody at (A) 0.5 and (B) 1 μg/mL.| Positive Control | 1) Cat. No. 1473 - Rat Cerebellum Tissue Lysate |
|---|---|
| Application Notes | RILPL1 antibody can be used for detection of RILPL1 by Western blot at 0.5 and 1 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | RILPL1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | RILPL1 Antibody is supplied in PBS containing 0.02% sodium azide. |
