Application
Immunohistochemistry of RIM2 in human brain tissue with RIM2 antibody at 5 μg/ml| Product Name | RIM2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RIM2 Antibody: OBOE, RIM2, RAB3IP3, KIAA0751, Regulating synaptic membrane exocytosis protein 2, Rab-3-interacting molecule 2, RIM 2 |
| Product description | RIM2 Antibody: Rab3-interacting molecules (RIMs) are synaptic proteins necessary for neuronal transmission and plasticity. Rim1 and Rim2 proteins are expressed in overlapping but distinct patterns throughout the brain. While the ablation of either gene was not lethal in mice, the deletion of both resulted in postnatal mortality. This lethality is due to a defect in neurotransmitter release; synapses without RIM proteins can still release neurotransmitters but are unable to do so in response to normal Ca2+ triggers. Like Rim1, Rim2 is thought to be an effector protein for Rab3, binding to Rab3 on synaptic vesicles in a GTP-dependent manner.1) Wang Y, Sugita S, and Sudhof TC. The RIM/NIM family of neuronal C2 domain proteins: interactions with Rab3 and a new class of Src homology 3 domain proteins. J. Biol. Chem. 2000; 275:20033-44. |
| Immunogen | RIM2 antibody was raised against a 17 amino acid synthetic peptide near the center of human RIM2. The immunogen is located within amino acids 840 - 890 of RIM2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-P |
| RIM2 antibody can be used for detection of RIM2 by immunohistochemistry at 5 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RIMS2 |
|---|---|
| Alternative Names | RIM2 Antibody: OBOE RIM2 RAB3IP3 KIAA0751 Regulating synaptic membrane exocytosis protein 2 Rab-3-interacting molecule 2 RIM 2 |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse: (100%) |
| Tissue Specificity | RIM2 antibody is human specific. Multiple isoforms of RIM2 are known to exist. |
| Entrez Gene | 9699 |
|---|---|
| Protein Accession | NP_001093587 |
Application
Immunohistochemistry of RIM2 in human brain tissue with RIM2 antibody at 5 μg/ml| Positive Control | 1) Cat. No. 10-301 - Human Brain Tissue Slide |
|---|---|
| Application Notes | RIM2 antibody can be used for detection of RIM2 by immunohistochemistry at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | RIM2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | RIM2 Antibody is supplied in PBS containing 0.02% sodium azide. |
