Application
Immunohistochemistry of Rim3 in human brain tissue with Rim3 antibody at 2.5 μg/mL.Application
Immunofluorescence of rim3 in human brain tissue with rim3 antibody at 5 μg/mL.Application
Western blot analysis of Rim3 in human brain tissue lysate with Rim3 antibody at 1 μg/mL in the (A) absence and (B) presence of blocking peptide.| Product Name | Rim3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Rim3 Antibody: NIM3, RIM3, KIAA0237, Regulating synaptic membrane exocytosis protein 3, RIM3 gamma, Nim3 |
| Product description | Rim3 Antibody: Rab3-interacting molecules (RIMs) are synaptic proteins are synaptic proteins necessary for neural transmission and plasticity. While both Rim1 and Rim 2 are thought to be effector proteins for Rab3, binding to Rab3 on synaptic vesicles in a GTP-dependent manner, less is known of Rim3. Expression of Rim3 in PC12 cells induced a significant increase in calcium-triggered exocytosis, with no appreciable change in the baseline release, suggesting that it plays a role in the regulation of exocytosis. Rim3 protein localizes primarily to neuronal dendrites and the postsynaptic densities, as opposed to Rim1 which is found in presynapse locations, indicating that Rim3 may contribute to synapse transmission and plasticity.1) Wang Y, Sugita S, and Sudhof TC. The RIM/NIM family of neuronal C2 domain proteins: interactions with Rab3 and a new class of Src homology 3 domain proteins. J. Biol. Chem.2000; 275:20033-44. |
| Immunogen | Rim3 antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of human Rim3. The immunogen is located within amino acids 60 - 110 of Rim3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Rim3 antibody can be used for detection of Rim3 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RIMS3 |
|---|---|
| Alternative Names | Rim3 Antibody: NIM3 RIM3 KIAA0237 Regulating synaptic membrane exocytosis protein 3 RIM3 gamma Nim3 |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Tissue Specificity | This antibody is predicted to have no cross-reactivity to other Rim proteins. |
Application
Immunohistochemistry of Rim3 in human brain tissue with Rim3 antibody at 2.5 μg/mL.
Application
Immunofluorescence of rim3 in human brain tissue with rim3 antibody at 5 μg/mL.
Application
Western blot analysis of Rim3 in human brain tissue lysate with Rim3 antibody at 1 μg/mL in the (A) absence and (B) presence of blocking peptide.| Positive Control | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
|---|---|
| Application Notes | Rim3 antibody can be used for detection of Rim3 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Rim3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Rim3 Antibody is supplied in PBS containing 0.02% sodium azide. |
