Application
Immunohistochemistry of RIP1 in human placenta tissue with RIP1 antibody at 10 μg/mL.| Product Name | RIP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RIP1 Antibody: RIP, RIP1, RIP, Cell death protein RIP, RIP-1 |
| Product description | RIP1 Antibody: RIP1 (Receptor Interacting Protein), also known as RIPK1, is a crucial 74 kD adaptor kinase in several of stress-induced signaling pathways and on the crossroad of a cell’s decision to live or die. RIP1 contains an N-terminal region with homology to protein kinases, an intermediate domain capable of association with MAPKKK and a C-terminal region containing a death domain motif present in the Fas and TNFR1 intracellular domains. Full length RIP1 is important for signallling to NF-kappa-B, MAPKs and necrosis, whereas caspase-8 generates a C-terminal RIP1 cleavage fragment, promoting TNF-induced apoptosis. It is required for TNFRSF1A-mediated and TLR3-induced NF-kappa-B activation. RIP1-deficient mice fail to thrive, displaying extensive apoptosis in both lymphoid and adipose tissues and dying at 1-3 days of age.1) Stanger BZ, Leder P, Lee TH, et al. RIP: a novel protein containing a death domain that interacts with Fas/APO-1 (CD95) in yeast and causes cell death. Cell 1995; 81:513-23. |
| Immunogen | RIP1 antibody was raised against a 14 amino acid peptide near the carboxy terminus of human RIP1. The immunogen is located within amino acids 600 - 650 of RIP1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-P |
| RIP1 antibody can be used for detection of RIP1 by immunohistochemistry at 5 - 10 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RIPK1 |
|---|---|
| Alternative Names | RIP1 Antibody: RIP RIP1 RIP Cell death protein RIP RIP-1 |
| Molecular Weight(MW) | Predicted: 74 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse: (100%) |
Application
Immunohistochemistry of RIP1 in human placenta tissue with RIP1 antibody at 10 μg/mL.| Application Notes | RIP1 antibody can be used for detection of RIP1 by immunohistochemistry at 5 - 10 μg/mL.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | RIP1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | RIP1 Antibody is supplied in PBS containing 0.02% sodium azide. |
Data sheet for OM290045