Application
Immunohistochemistry of RIPK1 in mouse kidney tissue with RIPK1 antibody at 2.5 μg/ml.Application
Immunohistochemistry of RIPK1 in mouse kidney tissue with RIPK1 antibody at 2.5 μg/mL.Application
Western blot analysis of RIPK1 in rat kidney tissue lysate with RIPK1 antibody at 1 μg/mL.Application
Immunofluorescence of RIPK1 in Mouse Kidney cells with RIPK1 antibody at 20 μg/mL.| Product Name | RIPK1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RIPK1 Antibody: RIP, RIP1, RIP, Cell death protein RIP, RIP-1 |
| Product description | RIPK1 Antibody: RIPK1 (Receptor Interacting Protein) is a crucial 74 kD adaptor kinase in several of stress-induced signaling pathways and on the crossroad of a cell's decision to live or die. RIPK1 contains an N-terminal region with homology to protein kinases, an intermediate domain capable of association with MAPKKK and a C-terminal region containing a death domain motif present in the Fas and TNFR1 intracellular domains. Full length RIPK1 is important for signallling to NFκ-B, MAPKs and necrosis, whereas caspase-8 generates a C-terminal RIPK1 cleavage fragment, promoting TNF-induced apoptosis. It is required for TNFRSF1A-mediated and TLR3-induced NF-κB activation. RIPK1-deficient mice fail to thrive, displaying extensive apoptosis in both lymphoid and adipose tissues and dying at 1-3 days of age.1) Stanger BZ, Leder P, Lee TH, et al. RIP: a novel protein containing a death domain that interacts with Fas/APO-1 (CD95) in yeast and causes cell death. Cell1995; 81:513-23. |
| Immunogen | RIPK1 antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human RIPK1. The immunogen is located within amino acids 180 - 230 of RIPK1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| WB:1:500~1:1000 IF:1:100~1:500 IHC-p:1:50~1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RIPK1 |
|---|---|
| Alternative Names | RIPK1 Antibody: RIP RIP1 RIP Cell death protein RIP RIP-1 |
Application
Immunohistochemistry of RIPK1 in mouse kidney tissue with RIPK1 antibody at 2.5 μg/ml.
Application
Immunohistochemistry of RIPK1 in mouse kidney tissue with RIPK1 antibody at 2.5 μg/mL.
Application
Western blot analysis of RIPK1 in rat kidney tissue lysate with RIPK1 antibody at 1 μg/mL.
Application
Immunofluorescence of RIPK1 in Mouse Kidney cells with RIPK1 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1465 - Rat Kidney Tissue Lysate |
|---|---|
| Application Notes | WB:1:500~1:1000 IF:1:100~1:500 IHC-p:1:50~1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | RIPK1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | RIPK1 Antibody is supplied in PBS containing 0.02% sodium azide. |
Data sheet for OM290051