Application
Immunohistochemistry of Rkhd1 in human small intestine tissue with Rkhd1 antibody at 2.5 μg/mL.Application
Immunofluorescence of rkhd1 in human small intestine tissue with rkhd1 antibody at 20 μg/mL.Application
Western blot analysis of Rkhd1 in MDA-MB-361 cell lysate with Rkhd1 antibody at (A) 1 μg/ml and (B) 2 μg/mL.| Product Name | Rkhd1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Rkhd1 Antibody: MEX3, TINO, RKHD1, MEX-3D, RNF193, OK/SW-cl.4, KIAA2031, RNA-binding protein MEX3D, RING finger and KH domain-containing protein 1 |
| Product description | Rkhd1 Antibody: Rkhd1, also known as TINO or MEX3D is a member of a novel family of four homologous human MEX3 proteins each containing two heterogeneous nuclear ribonucleoprotein K homology (KH) domains and one carboxy-terminal RING finger module. MEX3 proteins, including Rkhd1, are phosphoproteins that bind RNA through their KH domains and shuttle between the nucleus and the cytoplasm via the CRM1 export pathway. These proteins are a novel family of evolutionarily conserved RNA-binding proteins, differentially recruited to P bodies and potentially involved in post-transcriptional regulatory mechanisms. Rkhd1 binds to the AU-rich element in the 3'-untranslated region of Bcl-2 mRNA and is thought to be a negative regulator of Bcl-2 expression. Rkhd3 and Rkhd4, but not Rkhd1, co-localize with both the hDcp1a decapping factor and Argonaute (Ago) proteins in processing bodies (P bodies), recently characterized as centers of mRNA turnover.1) Donnini M, Lapucci A, Papucci L, et al. Identification of TINO: A new evolutionarily conserved Bcl-2 AU-rich element RNA-binding protein. J. Biol. Chem.2004; 279:20154-66. |
| Immunogen | Rkhd1 antibody was raised against a 15 amino acid synthetic peptide from near the center of human Rkhd1. The immunogen is located within amino acids 360 - 410 of Rkhd1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| Rkhd1 antibody can be used for detection of Rkhd1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MEX3D |
|---|---|
| Alternative Names | Rkhd1 Antibody: MEX3 TINO RKHD1 MEX-3D RNF193 OK/SW-cl.4 KIAA2031 RNA-binding protein MEX3D RING finger and KH domain-containing protein 1 |
| Sequence Similarities | 2) Cat. No. 11-801 - Human Small Intestine Tissue Slide |
Application
Immunohistochemistry of Rkhd1 in human small intestine tissue with Rkhd1 antibody at 2.5 μg/mL.
Application
Immunofluorescence of rkhd1 in human small intestine tissue with rkhd1 antibody at 20 μg/mL.
Application
Western blot analysis of Rkhd1 in MDA-MB-361 cell lysate with Rkhd1 antibody at (A) 1 μg/ml and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1217 - MDA-MB-361 Cell Lysate |
|---|---|
| Application Notes | Rkhd1 antibody can be used for detection of Rkhd1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Rkhd1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Rkhd1 Antibody is supplied in PBS containing 0.02% sodium azide. |
