Application
Immunofluorescence of RKHD3 in mouse skeletal muscle cells with RKHD3 antibody at 20 μg/mL.Application
Western blot analysis of RKHD3 in human testis tissue lysate with RKHD3 antibody at 1 μg/mL.| Product Name | RKHD3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RKHD3 Antibody: RKHD3, MEX-3B, RNF195, KIAA2009, RKHD3, RNA-binding protein MEX3B, RING finger and KH domain-containing protein 3 |
| Product description | RKHD3 Antibody: Rkhd3, also known as MEX3B is a member of a novel family of four homologous human MEX3 proteins each containing two heterogeneous nuclear ribonucleoprotein K homology (KH) domains and one carboxy-terminal RING finger module. MEX3 proteins, including Rkhd3, are phosphoproteins that bind RNA through their KH domains and shuttle between the nucleus and the cytoplasm via the CRM1 export pathway. These proteins are a novel family of evolutionarily conserved RNA-binding proteins, differentially recruited to P bodies and potentially involved in post-transcriptional regulatory mechanisms. Rkhd3 is thought to act as a mechanism to fine-tune mRNA regulation in early Xenopus development, and with Rkhd4, but not Rkhd1, will co-localize with both the hDcp1a decapping factor and Argonaute (Ago) proteins in processing bodies (P bodies), recently characterized as centers of mRNA turnover.1) Takada H, Kawana T, Ito Y, et al. The RNA-binding protein Mex3b has a fine-tuning system for mRNA regulation in early Xenopus development. Dev. 2009; 136:2413-22 |
| Immunogen | RKHD3 antibody was raised against a 16 amino acid synthetic peptide from near the center of human RKHD3. The immunogen is located within amino acids 180 - 230 of RKHD3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| RKHD3 antibody can be used for detection of EPAC1 by Western blot at 1 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MEX3B |
|---|---|
| Alternative Names | RKHD3 Antibody: RKHD3 MEX-3B RNF195 KIAA2009 RKHD3 RNA-binding protein MEX3B RING finger and KH domain-containing protein 3 |
| Molecular Weight(MW) | Predicted: 62 kDa Observed: 62 kDa |
| Tissue Specificity | This antibody is specific for RKHD3 and will not recognize the other RKHD3 family of proteins. |
Application
Immunofluorescence of RKHD3 in mouse skeletal muscle cells with RKHD3 antibody at 20 μg/mL.
Application
Western blot analysis of RKHD3 in human testis tissue lysate with RKHD3 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1313 - Human Testis Tissue Lysate |
|---|---|
| Application Notes | RKHD3 antibody can be used for detection of EPAC1 by Western blot at 1 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | RKHD3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | RKHD3 Antibody is supplied in PBS containing 0.02% sodium azide. |
