Application
Immunofluorescence of rkhd4 in human brain tissue with rkhd4 antibody at 20 μg/mL.Application
Immunohistochemistry of Rkhd4 in human brain tissue with Rkhd4 antibody at 2.5 μg/mL.Application
Western blot analysis of Rkhd4 in SK-N-SH cell lysate with Rkhd4 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | Rkhd4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Rkhd4 Antibody: RKHD4, MEX-3A, RKHD4, RNA-binding protein MEX3A, RING finger and KH domain-containing protein 4 |
| Product description | Rkhd4 Antibody: Rkhd4, also known as MEX3A is a member of a novel family of four homologous human MEX3 proteins each containing two heterogeneous nuclear ribonucleoprotein K homology (KH) domains and one carboxy-terminal RING finger module. MEX3 proteins, including Rkhd4, are phosphoproteins that bind RNA through their KH domains and shuttle between the nucleus and the cytoplasm via the CRM1 export pathway. These proteins are a novel family of evolutionarily conserved RNA-binding proteins, differentially recruited to P bodies and potentially involved in post-transcriptional regulatory mechanisms. While Rkhd2 has been suggested to be associated with susceptibility to essential hypertension type 8, the function of Rkhd4 remains unknown. Rkhd3 and Rkhd4, but not Rkhd2, co-localize with both the hDcp1a decapping factor and Argonaute (Ago) proteins in processing bodies (P bodies), recently characterized as centers of mRNA turnover.1) Draper BW, Mello CC, Bowerman B, et al. MEX-3 is a KH domain protein that regulates blastomere identity in early C. elegans embryos. Cell1996; 87:205-16. |
| Immunogen | Rkhd4 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human Rkhd4. The immunogen is located within amino acids 420 - 470 of Rkhd4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| Rkhd4 antibody can be used for detection of Rkhd4 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MEX3A |
|---|---|
| Alternative Names | Rkhd4 Antibody: RKHD4 MEX-3A RKHD4 RNA-binding protein MEX3A RING finger and KH domain-containing protein 4 |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Immunofluorescence of rkhd4 in human brain tissue with rkhd4 antibody at 20 μg/mL.
Application
Immunohistochemistry of Rkhd4 in human brain tissue with Rkhd4 antibody at 2.5 μg/mL.
Application
Western blot analysis of Rkhd4 in SK-N-SH cell lysate with Rkhd4 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1220 - SK-N-SH Cell Lysate |
|---|---|
| Application Notes | Rkhd4 antibody can be used for detection of Rkhd4 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Rkhd4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Rkhd4 Antibody is supplied in PBS containing 0.02% sodium azide. |
