Application
Immunohistochemistry of RLN3 in human brain tissue with RLN3 antibody at 5 μg/mL.Application
Western blot analysis of RLN3 in HeLa cell lysate with RLN3 antibody at (A) 1 and (B) 2 μg/ml.Application
Immunofluorescence of RLN3 in human brain tissue with RLN3 antibody at 20 μg/mL.| Product Name | RLN3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Relaxin-3, RXN3, H3, ZINS4, INSL7 |
| Product description | Relaxin-3 (RLN3) is a member of Relaxin family, a group of endocrine and autocrine/paracrine hormones that belong to the insulin gene superfamily (1,2). RLN3 can bind to and activate three relaxin-family peptide receptors: RXFP1, RXFP3, and RXFP4 (3). In contrast to the widespread expression of the related proteins RLN1 and RLN2, RLN3 is predominantly expressed in brain and to a lesser extent in the thymus, kidney and spleen (1). RLN3 has been suggested to play a role in feeding, metabolism, stress, arousal, learning, and memory (4).1) Bathgate RA, Samuel CS, Burazin TC, et al. Human relaxin gene 3 (H3) and the equivalent mouse relaxin (M3) gene. Novel members of the relaxin peptide family. J. Biol. Chem. 2002; 277:1148-57. |
| Immunogen | RLN3 antibody was raised against a 19 amino acid peptide near the center of human RLN3. The immunogen is located within amino acids 80 - 130 of RLN3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| RLN3 antibody can be used for detection of RLN3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RLN3 |
|---|---|
| Alternative Names | Relaxin-3 RXN3 H3 ZINS4 INSL7 |
| Molecular Weight(MW) | Predicted: 16 kDa Observed: 16 kDa |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Tissue Specificity | RLN3 antibody is human specific. RLN3 antibody is predicted to not cross-react with other members of the Relaxin protein family. |
Application
Immunohistochemistry of RLN3 in human brain tissue with RLN3 antibody at 5 μg/mL.
Application
Western blot analysis of RLN3 in HeLa cell lysate with RLN3 antibody at (A) 1 and (B) 2 μg/ml.
Application
Immunofluorescence of RLN3 in human brain tissue with RLN3 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
|---|---|
| Application Notes | RLN3 antibody can be used for detection of RLN3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | RLN3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | RLN3 antibody is supplied in PBS containing 0.02% sodium azide. |
