Application
Western blot analysis in HepG2 cell line lysates (35ug/lane).Application
RM33 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | RM33 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 39S ribosomal protein L33, mitochondrial, L33mt, MRP-L33, MRPL33, C2orf1 |
| Product description | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Alternatively spliced transcript variants encoding different isoforms have been described.1) Hillier, L.W., et al. Nature 434(7034):724-731(2005) |
| Immunogen | This RM33 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 36-65 amino acids from the C-terminal region of human RM33. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MRPL33 |
|---|---|
| Alternative Names | 39S ribosomal protein L33 mitochondrial L33mt MRP-L33 MRPL33 C2orf1 |
| Molecular Weight(MW) | 8 kDa |
Application
Western blot analysis in HepG2 cell line lysates (35ug/lane).
Application
RM33 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
