RNF12 is a RING-H2 zinc finger protein. It has been shown to be a ubiquitin protein ligase that targets LIM domain binding 1 (LDB1/CLIM), and causes proteasome-dependent degradation of LDB1. This protein and LDB1 are co-repressors of LHX1/LIM-1, a homeodomain transcription factor. Alternatively spliced transcript variants encoding the same protein have been reported.1) Hiratani, I., et al., (2003) Development 130 (17), 4161-4175.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RNF12.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAIHCWB
RNF12 antibody can be used for detection of RNF12 by ELISA at 1:12500. RNF12 antibody can be used for detection of RNF12 by western blot at 2.0 μ/mL.:
RNF12 antibody can be used for detection of RNF12 by ELISA at 1:12500. RNF12 antibody can be used for detection of RNF12 by western blot at 2.0 μ/mL.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store RNF12 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.