Application
Western blot analysis in ZR-75-1 cell line lysates (35ug/lane).| Product Name | RNF144B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, thereby promoting their degradation. Induces apoptosis via a TP53/p53-dependent but caspase-independent mechanism. However, its overexpression also produces a decrease of the ubiquitin-dependent stability of BAX, a pro-apoptotic protein, ultimately leading to protection of cell death; But, it is not an anti-apoptotic protein per se.1) Sayan, B.S., et al. Proc. Natl. Acad. Sci. U.S.A. 107(29):12877-12882(2010) |
| Immunogen | This RNF144B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 90-118 amino acids from the Central region of human RNF144B. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RNF144B |
|---|---|
| Alternative Names | E3 ubiquitin-protein ligase RNF144B 632- IBR domain-containing protein 2 RING finger protein 144B p53-inducible RING finger protein RNF144B IBRDC2 P53RFP |
| Molecular Weight(MW) | 34 kDa |
Application
Western blot analysis in ZR-75-1 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
