Application
Immunohistochemistry staining of RNF216 in liver tissue using RNF216 Antibody.| Product Name | RNF216 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | TRIADs are novel zinc finger proteins characterized by the presence of Cysteine rich TRIAD domain consisting of DRIL motif flanked by 2 RING fingers. TRIAD3, a 56 kDa protein, is a novel member of this family. It contains 4 RLD domains in the centre and a proline-rich domain at the C-terminus. Localized in the cytoplasm, it interacts with RIP and inhibits TNF and IL1-induced NF-kappaB activation pathways. Reports suggest TRIAD3 acts as an E3-ubiquitin-protein ligase and promotes ubiquitination and degradation of TLR4 and TLR9, thereby regulating TLR signaling. It also interacts with HIV type I Vif protein and interferes in the early events of HIV-1 replication. This gene encodes a cytoplasmic protein which specifically colocalizes and interacts with the serine/threonine protein kinase, receptor-interacting protein (RIP). Zinc finger domains of the encoded protein are required for its interaction with RIP and for inhibition of TNF- and IL1-induced NF-kappa B activation pathways. The encoded protein may also function as an E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes and transfers it to substrates. Several alternatively spliced transcript variants have been described for this locus but the full-length natures of only some are known. |
| Immunogen | RNF216 antibody was raised against amino acids 350 - 400 of RNF216 (Human). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| RNF216 antibody can be used in ELISA, Western Blot starting at 1:500 - 1:1000, and immunohistochemistry starting at 5 μg/mL. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | RNF216 |
|---|---|
| Alternative Names | RNF216 TRIAD3 UBCE7IP1 Ring finger protein 216 U7I1 ZIN |
Application
Immunohistochemistry staining of RNF216 in liver tissue using RNF216 Antibody.| Application Notes | RNF216 antibody can be used in ELISA, Western Blot starting at 1:500 - 1:1000, and immunohistochemistry starting at 5 μg/mL. |
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| Form | Liquid |
|---|---|
| Storage Instructions | RNF216 antibody can be stored short term 4 ˚C. For long term storage aliquot and store at -20 ˚C. As with all antibodies avoid freeze/thaw cycles. |
| Storage Buffer | PBS, 0.2% gelatin, 0.05% sodium azide. |
