Application
Antibody used in WB on Human HepG2 at 0.5 ug/ml.Application
Antibody used in IHC on Human Skin.| Product Name | RNF25 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RNF25, AO7, FLJ13906 |
| Product description | RNF25 contains a RING finger motif. The mouse counterpart of this protein has been shown to interact with Rela, the p65 subunit of NF-kappaB (NFKB), and modulate NFKB-mediated transcription activity. The mouse protein also binds ubiquitin-conjugating enzymes (E2s) and is a substrate for E2-dependent ubiquitination.The protein encoded by this gene contains a RING finger motif. The mouse counterpart of this protein has been shown to interact with Rela, the p65 subunit of NF-kappaB (NFKB), and modulate NFKB-mediated transcription activity. The mouse protein also binds ubiquitin-conjugating enzymes (E2s) and is a substrate for E2-dependent ubiquitination.1) Asamitsu, K., (2003) J. Biol. Chem. 278 (29), 26879-26887. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RNF25. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| RNF25 antibody can be used for detection of RNF25 by ELISA at 1:62500. RNF25 antibody can be used for detection of RNF25 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHuman |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RNF25 |
|---|---|
| Alternative Names | RNF25 AO7 FLJ13906 |
| Molecular Weight(MW) | 51 kDa |
Application
Antibody used in WB on Human HepG2 at 0.5 ug/ml.
Application
Antibody used in IHC on Human Skin.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | RNF25 antibody can be used for detection of RNF25 by ELISA at 1:62500. RNF25 antibody can be used for detection of RNF25 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store RNF25 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
