Application
Immunohistochemistry staining of RNF25 in cerebellum tissue using RNF25 Antibody.| Product Name | RNF25 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RNF25, AO7, Ring finger protein 25, Ring finger protein AO7 |
| Product description | RING finger protein 25 (RNF25, also named AO7) contains a RING finger domain and is ubiquitously expressed in various tissues. RNF25 was initially identified in a yeast two-hybrid screen of a murine T-cell library by using UbcH5b, an E2 enzyme, as bait. RNF25 has also been shown to act as a putative E3 ligase, at least in vitro. RNF25 localizes predominantly in the nucleus and supports the transcriptional activity of NF-κB by interacting with p65 in vivo upon stimulation with TNF. Yeast two-hybrid data also suggest that RNF25 interacts with a number of other molecules which may be potential ubiquitin ligase substrates. Among these are molecules that have critical roles in signal transduction and in regulation of translation (personal communication, Allan Weissman, CCR-NCI, Bethesda, MD). |
| Immunogen | RNF25 antibody was raised against amino acids 1 - 363 of RNF25 (Human) around the carboxy terminal. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCIPWB |
| RNF25 antibody can be used in ELISA, Western Blot, immunohistochemistry starting at 1:1000, immunofluorescence, and immunoprecipitation.: |
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| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RNF25 |
|---|---|
| Alternative Names | RNF25 AO7 Ring finger protein 25 Ring finger protein AO7 |
Application
Immunohistochemistry staining of RNF25 in cerebellum tissue using RNF25 Antibody.| Application Notes | RNF25 antibody can be used in ELISA, Western Blot, immunohistochemistry starting at 1:1000, immunofluorescence, and immunoprecipitation.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store RNF25 antibody at 4 ˚C or -20 ˚C. As with all antibodies avoid freeze/thaw cycles. |
| Storage Buffer | 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide. |
