RNF36 is specifically expressed in germ cells at round spermatid stages during spermatogenesis. The protein encoded by this gene contains an N-terminal RING finger motif, a B-box, and a C-terminal B-30.2-like domain. When overexpressed in COS-7 and HEK-293 cells, this protein was shown to induce cell apoptosis.1) Shyu, H.W., et al., (2003) Exp. Cell Res. 287 (2), 301-313.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RNF36.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
RNF36 antibody can be used for detection of RNF36 by ELISA at 1:12500. RNF36 antibody can be used for detection of RNF36 by western blot at 2.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
RNF36 antibody can be used for detection of RNF36 by ELISA at 1:12500. RNF36 antibody can be used for detection of RNF36 by western blot at 2.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store RNF36 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.