Application
Western blot analysis in mouse stomach tissue lysates (35ug/lane).Application
TRIM69 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | TRIM69 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | E3 ubiquitin-protein ligase TRIM69, 632-, RFP-like domain-containing protein trimless, RING finger protein 36, Tripartite motif-containing protein 69, TRIM69, RNF36 |
| Product description | This gene encodes a member of the RING-B-box-coiled-coil (RBCC) family and encodes a protein with an N-terminal RING finger motif, a PRY domain and a C-terminal SPRY domain. The mouse ortholog of this gene is specifically expressed in germ cells at the round spermatid stages during spermatogenesis and, when overexpressed, induces apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been described. It may play a role in apoptosis (By similarity).1) Shimada, M., et al. Hum. Genet. 128(4):433-441(2010) |
| Immunogen | This TRIM69 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 101-129 amino acids from the N-terminal region of human TRIM69. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TRIM69 |
|---|---|
| Alternative Names | E3 ubiquitin-protein ligase TRIM69 632- RFP-like domain-containing protein trimless RING finger protein 36 Tripartite motif-containing protein 69 TRIM69 RNF36 |
| Molecular Weight(MW) | 57 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat |
Application
Western blot analysis in mouse stomach tissue lysates (35ug/lane).
Application
TRIM69 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
