Application
Western blot analysis of RNF40 Antibody Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in HL-60 cell line lysate.| Product Name | RNF40 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | E3 ubiquitin-protein ligase BRE1B, BRE1-B, 632-, 95 kDa retinoblastoma-associated protein, RBP95, RING finger protein 40, RNF40, BRE1B, KIAA0661 |
| Product description | The protein encoded by this gene contains a RING finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein was reported to interact with the tumor suppressor protein RB1. Studies of the rat counterpart suggested that this protein may function as an E3 ubiquitin-protein ligase, and facilitate the ubiquitination and degradation of syntaxin 1, which is an essential component of the neurotransmitter release machinery.1) Chernikova, S.B., et al. Radiat. Res. 174(5):558-565(2010) |
| Immunogen | This RNF40 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 697-725 amino acids from the C-terminal region of human RNF40. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RNF40 |
|---|---|
| Alternative Names | E3 ubiquitin-protein ligase BRE1B BRE1-B 632- 95 kDa retinoblastoma-associated protein RBP95 RING finger protein 40 RNF40 BRE1B KIAA0661 |
| Molecular Weight(MW) | 114 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Western blot analysis of RNF40 Antibody Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in HL-60 cell line lysate.| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
