Application
Antibody used in WB on Human MCF-7 at 0.2-1 ug/ml.| Product Name | RNF43 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RNF43, DKFZp781H02126, DKFZp781H0392, FLJ20315, MGC125630, RNF124, URCC |
| Product description | RNF43 is a HAP95 (AKAP8L) binding ubiquitin ligase that promotes cell growth and is upregulated in colon cancer.1) Sugiura, T., (2008) Exp. Cell Res. 314 (7), 1519-1528. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RNF43. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| RNF43 antibody can be used for detection of RNF43 by ELISA at 1:312500. RNF43 antibody can be used for detection of RNF43 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RNF43 |
|---|---|
| Alternative Names | RNF43 DKFZp781H02126 DKFZp781H0392 FLJ20315 MGC125630 RNF124 URCC |
| Molecular Weight(MW) | 86 kDa |
Application
Antibody used in WB on Human MCF-7 at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1219 - MCF7 Cell Lysate |
|---|---|
| Application Notes | RNF43 antibody can be used for detection of RNF43 by ELISA at 1:312500. RNF43 antibody can be used for detection of RNF43 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store RNF43 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
