Application
RNF43 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis in CEM cell line lysates (35ug/lane).Application
Western Blot at 1:2000 dilution + A549 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | RNF43 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | E3 ubiquitin-protein ligase RNF43, 632-, RING finger protein 43, RNF43 |
| Product description | RNF43 is a HAP95 (AKAP8L; MIM 609475) binding ubiquitin ligase that promotes cell growth and is upregulated in colon cancer (Yagyu et al., 2004 [PubMed 15492824]; Sugiura et al., 2008 [PubMed 18313049]).1) Low, S.K., et al. PLoS ONE(7), E11824 (2010) : |
| Immunogen | This RNF43 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 543-572 amino acids from the C-terminal region of human RNF43. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RNF43 |
|---|---|
| Alternative Names | E3 ubiquitin-protein ligase RNF43 632- RING finger protein 43 RNF43 |
| Molecular Weight(MW) | 86 kDa |
Application
RNF43 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis in CEM cell line lysates (35ug/lane).
Application
Western Blot at 1:2000 dilution + A549 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
