Application
RNF5 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis of lysate from K562 cell line, using RNF5 Antibody at 1:1000 at each lane.| Product Name | RNF5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | E3 ubiquitin-protein ligase RNF5, 632-, Protein G16, RING finger protein 5, Ram1 homolog, HsRma1, RNF5, G16, NG2, RMA1 |
| Product description | The protein encoded by this gene contains a RING finger, which is a motif known to be involved in protein-protein interactions. This protein is a membrane-bound ubiquitin ligase. It can regulate cell motility by targeting paxillin ubiquitination and altering the distribution and localization of paxillin in cytoplasm and cell focal adhesions.1) Barcellos, L.F., et al. PLoS Genet.(10), E1000696 (2009) : |
| Immunogen | This RNF5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 65-94 amino acids from the Central region of human RNF5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RNF5 |
|---|---|
| Alternative Names | E3 ubiquitin-protein ligase RNF5 632- Protein G16 RING finger protein 5 Ram1 homolog HsRma1 RNF5 G16 NG2 RMA1 |
| Molecular Weight(MW) | 20 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
RNF5 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis of lysate from K562 cell line, using RNF5 Antibody at 1:1000 at each lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
