RNMT belongs to the mRNA cap methyltransferase family. RNMT is a mRNA-capping methyltransferase that methylates the N7 position of the added guanosine to the 5'-cap structure of mRNAs. It binds RNA containing 5'-terminal GpppC.1) Shafer, B., (2005) Mol. Cell. Biol. 25 (7), 2644-2649.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RNMT.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
RNMT antibody can be used for detection of RNMT by ELISA at 1:312500. RNMT antibody can be used for detection of RNMT by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody used in WB on Human NTERA2 at 0.2-1 ug/ml.
Positive Control
1) NTERA2 Cell Lysate
Application Notes
RNMT antibody can be used for detection of RNMT by ELISA at 1:312500. RNMT antibody can be used for detection of RNMT by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store RNMT antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.