Application
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis in 293,K562,HL-60 cell line lysates (35ug/lane).Application
Roundabout 4 Antibody IHC analysis in formalin fixed and paraffin embedded prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Roundabout 4 Antibody for immunohistochemistry.| Product Name | Roundabout 4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Roundabout homolog 4, Magic roundabout, ROBO4 |
| Product description | Roundabout 4 is receptor for Slit proteins, at least for SLIT2, and seems to be involved in angiogenesis and vascular patterning. This protein may mediate the inhibition of primary endothelial cell migration by Slit proteins.1) Jones, C.A., et al. Nat. Cell Biol. 11(11):1325-1331(2009) |
| Immunogen | This Roundabout 4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 236-264 amino acids from the N-terminal region of human Roundabout 4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ROBO4 |
|---|---|
| Alternative Names | Roundabout homolog 4 Magic roundabout ROBO4 |
| Molecular Weight(MW) | 107 kDa |
Application
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis in 293,K562,HL-60 cell line lysates (35ug/lane).
Application
Roundabout 4 Antibody IHC analysis in formalin fixed and paraffin embedded prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Roundabout 4 Antibody for immunohistochemistry.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
