Application
Immunohistochemistry of RP105 in human spleen tissue withRP105 antibody at 10 μg/mL.Application
Western blot analysis of RP105 in human spleen tissue lysate with RP105 antibody at (A) 0.5 and (B) 1 μg/mL.| Product Name | RP105 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RP105 Antibody: LY64, Ly78, RP105, LY64, CD180 antigen, Lymphocyte antigen 64 |
| Product description | RP105 Antibody: Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. The signaling of these TLRs is kept under tight control by the expression of endogenous inhibiting proteins. One such protein is RP105, a recently identified homolog to TLR4 that, with MD-1, interacts with and inhibits the TLR4/MD-2 signaling pathway. It has also been suggested that the RP105/MD-1 complex influences antibody production mediated by both TLR4/MD-2 and TLR2 receptor complexes.1) Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol. 2003; 21:335-76. |
| Immunogen | RP105 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human RP105. The immunogen is located within amino acids 590 - 640 of RP105. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| RP105 antibody can be used for the detection of RP105 by Western blot at 0.5 - 1 μg/mL. Despite its predicted molecular weight, RP105 often migrates at 95 - 105 kDa. Antibody can also be used for immunohistochemistry starting at 10 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CD180 |
|---|---|
| Alternative Names | RP105 Antibody: LY64 Ly78 RP105 LY64 CD180 antigen Lymphocyte antigen 64 |
| Sequence Similarities | 2) Cat. No. 10-901 - Human Spleen Tissue Slide |
Application
Immunohistochemistry of RP105 in human spleen tissue withRP105 antibody at 10 μg/mL.
Application
Western blot analysis of RP105 in human spleen tissue lysate with RP105 antibody at (A) 0.5 and (B) 1 μg/mL.| Positive Control | 1) Cat. No. 1306 - Human Spleen Tissue Lysate |
|---|---|
| Application Notes | RP105 antibody can be used for the detection of RP105 by Western blot at 0.5 - 1 μg/mL. Despite its predicted molecular weight, RP105 often migrates at 95 - 105 kDa. Antibody can also be used for immunohistochemistry starting at 10 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | RP105 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | RP105 Antibody is supplied in PBS containing 0.02% sodium azide. |
