Application
Immunohistochemistry of RPA Interacting Protein in mouse stomach tissue with RPA Interacting Protein antibody at 2.5 μg/mL.Application
Western blot analysis of RPA Interacting Protein in Jurkat cell lysate with RPA Interacting Protein antibody at (A) 1 and (B) 2 μg/mL.| Product Name | RPA Interacting Protein Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | RPA Interacting Protein Antibody: Replication protein A (RPA) is a single-stranded-DNA binding protein involved in numerous eukaryotic DNA processes including replication, repair and recombination. RPA interacting protein (RPA IP) has been identified as an adapter protein that is involved in RPA nuclear import instead of the prototypical importin proteins that normally mediate nuclear import. Multiple isoforms of RPA IP are known to exist, with the longest isoform localized to the cytoplasm. Isoform 2 is sumoylated and is located in the PML nuclear body within the nucleus. It has been suggested that this isoform mediates the localization of the RPA complex into the PML nuclear body, thereby participating in RPA function in DNA metabolism.1) Zou Y, Liu Y, Wu X, et al. Functions of human replication protein A (RPA): from DNA replication to DNA damage and stress responses. J. Cell Physiol. 2006; 208:267-73. |
| Immunogen | RPA IP antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human RPA IP. The immunogen is located within the last 50 amino acids of RPA Interacting Protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-P |
| RPA Interacting Protein antibody can be used for detection of RPA Interacting Protein by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. | |
| Species Reactivity | Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RPAIN |
|---|---|
| Alternative Names | RPA Interacting Protein Antibody: RIP HRIP RIP RPA-interacting protein hRIP |
| Sequence Similarities | 2) Cat. No. 1415 - Mouse Stomach Tissue Lysate |
| Entrez Gene | 84268 |
|---|---|
| Protein Accession | NP_001028174 |
Application
Immunohistochemistry of RPA Interacting Protein in mouse stomach tissue with RPA Interacting Protein antibody at 2.5 μg/mL.
Application
Western blot analysis of RPA Interacting Protein in Jurkat cell lysate with RPA Interacting Protein antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | RPA Interacting Protein antibody can be used for detection of RPA Interacting Protein by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | RPA Interacting Protein antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | RPA Interacting Protein Antibody is supplied in PBS containing 0.02% sodium azide. |
